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pam6  (ATCC)


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    Structured Review

    ATCC pam6
    Pam6, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+pam6/AM6+purified+plasmid+DNA/pmc08588301__gutjnl___2020___323300supp002-17-9-10
    Average 93 stars, based on 22 article reviews
    pam6 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Compositions and methods for treating intracellular diseases
    Article Snippet: .. A 1.8 Kb BamH I fragment containing the entire precore/core coding region of hepatitis B is obtained from plasmid pAM6 (ATCC No 45020) and ligated into the BamH I site of KS II+ (Stratagene, La Jolla, Calif.). .. The mixture is vortexed and then centrifuged 14,000 rpm for 5 minutes in a Brinkmann Eppendorf centrifuge (5415L).

    Article Title: RNA detection assays
    Article Snippet: .. This material was created using the polymerase chain reaction from plasmid pAM6 (ATCC #45020D). .. The PCRs were conducted using a vector-based forward primer, oligo # 156-022-001 (5′-ggcgaccacacccgtcctgt-3′; SEQ ID NO:594) and a reverse primer, oligo #156-022-02 (5′-ccacgatgcgtccggcgtag-3′; SEQ ID NO:595) to amplify the full length of the HBV insert, an amplicon of about 3.2 kb.

    Article Title: Reactions on a solid surface
    Article Snippet: .. This material was created using the polymerase chain reaction from plasmid pAM6 (ATCC #45020D). .. The PCRs were conducted using a vector-based forward primer, oligo # 156-022-001 (5′-ggcgaccacacccgtcctgt-3′; SEQ ID NO:168) and a reverse primer, oligo #156-022-02 (5′-ccacgatgcgtccggcgtag-3′; SEQ ID NO:169) to amplify the full length of the HBV insert, an amplicon of about 3.2 kb.

    Article Title: RNA detection assays
    Article Snippet: .. This material was created using the polymerase chain reaction from plasmid pAM6 (ATCC #45020D). .. The PCRs were conducted using a vector-based forward primer, oligo # 156-022-001 (5′-ggcgaccacacccgtcctgt-3′; SEQ ID NO:594) and a reverse primer, oligo #156-022-02 (5′-ccacgatgcgtccggcgtag-3′; SEQ ID NO:595) to amplify the full length of the HBV insert, an amplicon of about 3.2 kb.

    Article Title: Quantification of intrahepatic total HBV DNA in liver biopsies of HBV-infected patients by a modified version of COBAS ® Ampliprep/COBAS ® TaqMan HBV test v2.0.
    Article Snippet: .. Before testing the liver biopsies, the linearity and reproducibility of the modified protocol was first tested by using serial dilutions of the full-length HBV genome-encoding plasmid pAM6 (ATCC® 45020DTM ATCC, Manassas, VA), diluted at the concentrations of 6.3 log IU, 5.3 log IU, 4.3 log IU, 3.3 log IU, 2.3 log IU, 1.3 log IU and 5 IU. ..

    Article Title: Total and covalently closed circular DNA detection in liver tissue of long-term survivors transplanted for HBV-related cirrhosis.
    Article Snippet: Background: Life-long prophylaxis against HBV recurrence is recommended in patients transplanted for HBV-related disease.. The risk of HBV reactivation is due to persistence of covalently closed circular (ccc) DNA in hepatocytes.. Whether cccDNA persists in livers of long-term transplant survivors who received conventional prophylaxis is unknown.

    Article Title: Eukaryotic layered vector initiation systems
    Article Snippet: .. Isolation of HBV E/Core Sequence A 1.8 Kb fragment containing the entire precore/core coding region of hepatitis B is obtained from plasmid pAM6 (ATCC No 45020) following Bam HI digestion and gel purification, and ligated into the Bam HI site of KS II+(Stratagene, La Jolla, Calif.). ..

    Article Title: Change of hepatitis B virus DNA and covalently closed circular DNA status in liver graft after liver transplantation.
    Article Snippet: Transplantat ABSTRACT Recurrence of hepatitis B virus (HBV) occurs despite prophylaxis, and covalently closed circular DNA (cccDNA) is thought to play a role owing to its resistance to prophylactic agents used.. The aim of this study was to evaluate the changes of HBV DNA and cccDNA within the liver graft during liver transplantation (LT).. Polymerase chain reaction (PCR) primers and probes were designed to measure total HBV DNA (tDNA) and cccDNA by real-time PCR.

    Polymerase Chain Reaction:

    Article Title: RNA detection assays
    Article Snippet: .. This material was created using the polymerase chain reaction from plasmid pAM6 (ATCC #45020D). .. The PCRs were conducted using a vector-based forward primer, oligo # 156-022-001 (5′-ggcgaccacacccgtcctgt-3′; SEQ ID NO:594) and a reverse primer, oligo #156-022-02 (5′-ccacgatgcgtccggcgtag-3′; SEQ ID NO:595) to amplify the full length of the HBV insert, an amplicon of about 3.2 kb.

    Article Title: Reactions on a solid surface
    Article Snippet: .. This material was created using the polymerase chain reaction from plasmid pAM6 (ATCC #45020D). .. The PCRs were conducted using a vector-based forward primer, oligo # 156-022-001 (5′-ggcgaccacacccgtcctgt-3′; SEQ ID NO:168) and a reverse primer, oligo #156-022-02 (5′-ccacgatgcgtccggcgtag-3′; SEQ ID NO:169) to amplify the full length of the HBV insert, an amplicon of about 3.2 kb.

    Article Title: RNA detection assays
    Article Snippet: .. This material was created using the polymerase chain reaction from plasmid pAM6 (ATCC #45020D). .. The PCRs were conducted using a vector-based forward primer, oligo # 156-022-001 (5′-ggcgaccacacccgtcctgt-3′; SEQ ID NO:594) and a reverse primer, oligo #156-022-02 (5′-ccacgatgcgtccggcgtag-3′; SEQ ID NO:595) to amplify the full length of the HBV insert, an amplicon of about 3.2 kb.

    Modification:

    Article Title: Quantification of intrahepatic total HBV DNA in liver biopsies of HBV-infected patients by a modified version of COBAS ® Ampliprep/COBAS ® TaqMan HBV test v2.0.
    Article Snippet: .. Before testing the liver biopsies, the linearity and reproducibility of the modified protocol was first tested by using serial dilutions of the full-length HBV genome-encoding plasmid pAM6 (ATCC® 45020DTM ATCC, Manassas, VA), diluted at the concentrations of 6.3 log IU, 5.3 log IU, 4.3 log IU, 3.3 log IU, 2.3 log IU, 1.3 log IU and 5 IU. ..

    Control:

    Article Title: Total and covalently closed circular DNA detection in liver tissue of long-term survivors transplanted for HBV-related cirrhosis.
    Article Snippet: Background: Life-long prophylaxis against HBV recurrence is recommended in patients transplanted for HBV-related disease.. The risk of HBV reactivation is due to persistence of covalently closed circular (ccc) DNA in hepatocytes.. Whether cccDNA persists in livers of long-term transplant survivors who received conventional prophylaxis is unknown.

    Construct:

    Article Title: Total and covalently closed circular DNA detection in liver tissue of long-term survivors transplanted for HBV-related cirrhosis.
    Article Snippet: Background: Life-long prophylaxis against HBV recurrence is recommended in patients transplanted for HBV-related disease.. The risk of HBV reactivation is due to persistence of covalently closed circular (ccc) DNA in hepatocytes.. Whether cccDNA persists in livers of long-term transplant survivors who received conventional prophylaxis is unknown.

    Real-time Polymerase Chain Reaction:

    Article Title: Total and covalently closed circular DNA detection in liver tissue of long-term survivors transplanted for HBV-related cirrhosis.
    Article Snippet: Background: Life-long prophylaxis against HBV recurrence is recommended in patients transplanted for HBV-related disease.. The risk of HBV reactivation is due to persistence of covalently closed circular (ccc) DNA in hepatocytes.. Whether cccDNA persists in livers of long-term transplant survivors who received conventional prophylaxis is unknown.

    Isolation:

    Article Title: Eukaryotic layered vector initiation systems
    Article Snippet: .. Isolation of HBV E/Core Sequence A 1.8 Kb fragment containing the entire precore/core coding region of hepatitis B is obtained from plasmid pAM6 (ATCC No 45020) following Bam HI digestion and gel purification, and ligated into the Bam HI site of KS II+(Stratagene, La Jolla, Calif.). ..

    Sequencing:

    Article Title: Eukaryotic layered vector initiation systems
    Article Snippet: .. Isolation of HBV E/Core Sequence A 1.8 Kb fragment containing the entire precore/core coding region of hepatitis B is obtained from plasmid pAM6 (ATCC No 45020) following Bam HI digestion and gel purification, and ligated into the Bam HI site of KS II+(Stratagene, La Jolla, Calif.). ..

    Gel Purification:

    Article Title: Eukaryotic layered vector initiation systems
    Article Snippet: .. Isolation of HBV E/Core Sequence A 1.8 Kb fragment containing the entire precore/core coding region of hepatitis B is obtained from plasmid pAM6 (ATCC No 45020) following Bam HI digestion and gel purification, and ligated into the Bam HI site of KS II+(Stratagene, La Jolla, Calif.). ..

    Amplification:

    Article Title: Change of hepatitis B virus DNA and covalently closed circular DNA status in liver graft after liver transplantation.
    Article Snippet: Transplantat ABSTRACT Recurrence of hepatitis B virus (HBV) occurs despite prophylaxis, and covalently closed circular DNA (cccDNA) is thought to play a role owing to its resistance to prophylactic agents used.. The aim of this study was to evaluate the changes of HBV DNA and cccDNA within the liver graft during liver transplantation (LT).. Polymerase chain reaction (PCR) primers and probes were designed to measure total HBV DNA (tDNA) and cccDNA by real-time PCR.

    Quantitative Proteomics:

    Article Title: Change of hepatitis B virus DNA and covalently closed circular DNA status in liver graft after liver transplantation.
    Article Snippet: Transplantat ABSTRACT Recurrence of hepatitis B virus (HBV) occurs despite prophylaxis, and covalently closed circular DNA (cccDNA) is thought to play a role owing to its resistance to prophylactic agents used.. The aim of this study was to evaluate the changes of HBV DNA and cccDNA within the liver graft during liver transplantation (LT).. Polymerase chain reaction (PCR) primers and probes were designed to measure total HBV DNA (tDNA) and cccDNA by real-time PCR.



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    Fig. 1 a The graph shows the linear relationship between the expected and the observed data of plasmid <t>pAM6.</t> b The histogram reports the mean (SD) concentration of pAM6 obtained in the first (light grey) and second (dark grey) run. In particular, each expected concentration of pAM6 was tested in triplicate in two different runs, and the mean (SD) of values obtained was then calculated. The val- ues obtained testing the STD concentration of 1.3 log IU were not included in the graph because some of them fell below the limit of quantification of the assay
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    Fig. 1 a The graph shows the linear relationship between the expected and the observed data of plasmid <t>pAM6.</t> b The histogram reports the mean (SD) concentration of pAM6 obtained in the first (light grey) and second (dark grey) run. In particular, each expected concentration of pAM6 was tested in triplicate in two different runs, and the mean (SD) of values obtained was then calculated. The val- ues obtained testing the STD concentration of 1.3 log IU were not included in the graph because some of them fell below the limit of quantification of the assay
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    Image Search Results


    Fig. 1 a The graph shows the linear relationship between the expected and the observed data of plasmid pAM6. b The histogram reports the mean (SD) concentration of pAM6 obtained in the first (light grey) and second (dark grey) run. In particular, each expected concentration of pAM6 was tested in triplicate in two different runs, and the mean (SD) of values obtained was then calculated. The val- ues obtained testing the STD concentration of 1.3 log IU were not included in the graph because some of them fell below the limit of quantification of the assay

    Journal: Medical microbiology and immunology

    Article Title: Quantification of intrahepatic total HBV DNA in liver biopsies of HBV-infected patients by a modified version of COBAS ® Ampliprep/COBAS ® TaqMan HBV test v2.0.

    doi: 10.1007/s00430-017-0504-3

    Figure Lengend Snippet: Fig. 1 a The graph shows the linear relationship between the expected and the observed data of plasmid pAM6. b The histogram reports the mean (SD) concentration of pAM6 obtained in the first (light grey) and second (dark grey) run. In particular, each expected concentration of pAM6 was tested in triplicate in two different runs, and the mean (SD) of values obtained was then calculated. The val- ues obtained testing the STD concentration of 1.3 log IU were not included in the graph because some of them fell below the limit of quantification of the assay

    Article Snippet: Before testing the liver biopsies, the linearity and reproducibility of the modified protocol was first tested by using serial dilutions of the full-length HBV genome-encoding plasmid pAM6 (ATCC® 45020DTM ATCC, Manassas, VA), diluted at the concentrations of 6.3 log IU, 5.3 log IU, 4.3 log IU, 3.3 log IU, 2.3 log IU, 1.3 log IU and 5 IU.

    Techniques: Plasmid Preparation, Concentration Assay